The problem of soil erosion and desertification in Tibet Plateau has been aggravated due to global warming and human impacts in recent decades. In this study, a case study of Amdo County was carried out using high resolution sampling to investigate how soil properties changed by desertification in Tibet Plateau. The results indicated that soil water retention and soil resistance to wind erosion decreased during the desertification transformation from potential to severe. Meanwhile, soil pH increased dramatically and soil particles become coarse. Nutrient deficiency was a common feature in the desertified grasslands, which should be paid more attention on the early desertification prevention. In addition, some suggestions also were put forward for the vegetation restoration in terms of the soil evolutional characteristics in Tibet Plateau.