In order to investigate the effects of nighttime warming and no-tillage on soybean growth and its uptake and utilization of nitrogen (N) and phosphorus (P),field nighttime warming system was designed in trial field in Nanjing University of Information Science and Technology. The experiments were conducted with four treatments,that is,normal nighttime temperature with traditional tillage (CK),nighttime warming with traditional tillage (W),normal nighttime temperature with no-tillage (NT),and nighttime warming with no-tillage (WNT). The results showed that compared to CK,nighttime warming (W) significantly decreased plant biomass by 6%-25% during the growing season (P < 0. 05),whereas had little effect on grain yield,grains per plant,grain weight per plant,and 100-seed weight of soybean. During branching and flowering stages,warming reduced N accumulation by 30% and 20%,respectively,and P accumulation by 39% and 39%. No-tillage had a limited effect on grain yield and yield components,but significantly decreased plant biomass,translocation amounts and efficiencies of N and P (P < 0. 01). Warming with no-tillage(WNT) treatment significantly decreased plant biomass and grain yield by 29% and 34%,respectively (P < 0. 01). Moreover,grains per plant,grain weight per plant,and 100-seed eight of soybean were reduced by 22%,22% and 3%,respectively,under WNT treatment. Meanwhile,at maturity,N and P accumulations were 20% and 22% lower under WNT treatment than under CK treatment,respectively. Thus,this study indicated that grain yield and yield components of soybean were hardly affected by single factor like either nighttime warming or no-tillage,whereas nighttime warming with no-tillage significantly reduced grain yield of soybean and N and P accumulation. Therefore,it is unfavorable to soybean production by means of no-tillage under climate warming condition.