Whether palaeolakes were widely distributed in Northwestern China during Marine Isotope Stage 3 (MIS 3) is still under debate. Radiocarbon dating data suggested that the paleolakes developed during MIS 3. However, the Optically Stimulated Luminescence (OSL) dating data have indicated that the ages of paleolakes ~(14)C dating dated to MIS 3 are mostly fall in MIS 5. Ejina Basin is a terminal lake basin of the Heihe River, consisting of three sub-basins of Gaxun Nur, Sogo Nur and Juyanze from east to west. The basin is surrounded by Gobi Altay Mountains in the north,Badain Jaran desert in the southeast, and alluvial fans of Heihe River in the south. The ~(14)C dating of palaeoshorelines at the Ejina Basin have suggested that megalake appeared during some time of MIS 3,however, this is still lack of lacustrine records studies from central of the basin as nearly all lacustrine records are just focused on Holocene paleoenvironmental/ climatic changes. In this study, we drilled and retrieved a 61. 13m deep core JYZ11A (41053'41. 4"N, 101°51 '05. 4"E; 895m a.s.l.) at the central of Juyanze Basin. Environmental proxy index samples were collected at 2cm interval in the upper 10m and 5cm intervals from 10. 00~61. 13m of the drill core and only upper 27m of the drill core are presented in this study. According to lithology and sedimentary, the upper 27m of the drill core sequence can be divided into two stratigraphical units from bottom to top. Unit I (27. 00 ~ 8. 88m) is reddish brown coarse sand or yellowish brown sand, and Unit Ⅱ (8. 88m ~ 0) is grey silty clay alternating with fine-to-medium sand. Three OSL samples were collected at the sand layer of the drill core at the depth of ca.6. 2m, 9. 3m and 22. 2m in the darkroom, and the ages of these three samples are 3. 00. 2ka,6. 70. 6ka and 80. 48. 4ka,respectively. A total of 508 samples at 10cm interval of JYZ11A core were collected for grain size analysis including different grain size fractions and frequency distribution for typical samples, and a total of 463 samples at 2cm interval of upper 16. 7m of JYZ11A core were collected for low frequency magnetic susceptibility (chi_(lf)) analysis. Combining lithology, grain size frequency distribution for typical samples from upper 27m of JYZ11A core and previously published proxy indexes results of JYZ11A core, it is suggested that an aridity environment characterized by prevailing alluvium sands and eolian sands appeared in Juyanze Basin during the Last Glaciation and Early Holocene. Shallow lake environment with frequent wind-sand activities appeared only after ca.6. 7ka and the stable lake environment occurred in the region after ca.3ka. We suggest that no permanent paleolake appeared in Juyanze area during the MIS 3 and even Last Glaciation. The evolutionary pattern of lake in Juyanze Basin during Holocene presents a difference comparing to the lake evolution in monsoonal China.