Selection of heat-resistance cultivars is an important approach for coping with climate changes as well as providing heat tolerant germplasm for wheat breeding. Thirteen wheat (Triticum aestivum L.) cultivars and advanced lines collected from the Northern China Winter Wheat Region and Huanghuai Winter Wheat Region were planted in Shijiazhuang in 2013-2014 cropping season to investigate the effects of heat stress on yield and grain quality. The results showed that thousand-kernel weight (TKW) might be used as a simple criterion in selection for heat-resistance. Zhongmai 895, CA0816, Heng 4444, Jingdong 8 and Shimai 15 obtained high yield and high TKW under both normal and heat-stress environments,and were characterized as high resistance to heat stress; Zhongmai 875 achieved medium yield but high TKW in all environments,and were characterized as good resistance to heat stress; Heng08guan 29, Hengguan 35 and Shiyou 17 obtained high yield and high TKW in normal environment, but low yield and low TKW in heat stress environment, and were characterized as poor resistance to heat stress. The grain hardness index was increased under heat stress environment,and other indexes showed different responses. The water absorption, wet gluten and grain hardness of Shiyou 17 showed significant differences under normal and heat stress environments. The grain protein content, extensibility and wet gluten of Heng08guan 29 also showed significant difference under normal and heat stress environment. The grain filling rate reached 1.5 ~1.8 g?d~(-1) under normal environment compared with 1.3~1.6 g?d~(-1) under heat stress environment.