globalchange  > 气候变化与战略
CSCD记录号: CSCD:5419812
论文题名:
高温刺激对蟾蜍免疫活性和能量消耗的影响
其他题名: The effects of hyperthermia on immunocompetence and energy consumption in Bufo bufo gargarizans
作者: 肖锋; 颉志刚; 庄建洲
刊名: 生态学报
ISSN: 1000-0933
出版年: 2015
卷: 35, 期:9, 页码:159-166
语种: 中文
中文关键词: 中华大蟾蜍 ; 高温刺激 ; 免疫活性 ; 能量消耗
英文关键词: Bufo bufo gargarizan ; hyperthermia ; immunocompetence ; energy consumption
WOS学科分类: BIOLOGY
WOS研究方向: Life Sciences & Biomedicine - Other Topics
中文摘要: 全球气候变化是导致全世界两栖动物种群衰退和灭绝的重要因素之一。为了解无尾两栖类在气候异常导致的极端高温下的生理反应特征和相关适应机制,以雄性中华大蟾蜍(Bufo bufo gargarizan)为对象,研究该物种在高温刺激(环境温度由22 ℃迅速升至32 ℃)0、6、12、24、48 h后的非特异性免疫活性和能量消耗的变化情况。研究结果:与对照组(22 ℃ )比较,高温刺激可显著抑制外周血细胞吞噬活性;而脾脏巨噬细胞呼吸爆发在高温刺激下呈现出先增强后减弱的趋势。高温刺激导致血糖含量在第24和48 h显著低于对照组,而对肝糖原含量无显著影响。处理组精巢ATP含量逐渐升高,并于24和48 h显著高于对照组;处理组Na~+-K+-ATP_(ase)活性随时间呈现出先下降后上升稳定的趋势,但与对照组相比均无显著性差异,;处理组Ca~(2+)-Mg~(2+)-ATPase活性在不同时期与对照组相比均无显著性差异。研究结果说明,高温刺激可显著抑制中华大蟾蜍的非特异性免疫功能,并导致血糖的大量消耗;当面对高温胁迫时,雄性大蟾蜍可能会投入更多的能量来维持性腺的正常发育。
英文摘要: Global climate change is a key factor which leads to either a decline or an extinction of amphibian populations worldwide. This paper investigates the effects of high environmental temperatures on non-specific immunity and energy consumption in the male toads (Bufo bufo gargarizans). We found that peripheral blood cell phagocytosis decreases while spleen macrophage respiratory burst activity transiently increases in high-temperature toads (32 ℃) versus control toads (22 ℃). Blood glucose levels decrease in high-temperature toads after 24 h and 48 h exposure versus control toads, while hepatic glycogen content remains unchanged. In addition, the ATP content in the high-temperature toad testis gradually increases and reaches significant levels after 24 h and 48 h exposure versus control toads. Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase activity show no significant changes. Our results indicate that high environmental temperatures cause a decline in immunocompetence,an increase in glucose consumption,and an accumulation of energy (ATP) within the testis in Bufo bufo gargarizans.
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/149802
Appears in Collections:气候变化与战略

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作者单位: 浙江师范大学生态研究所, 金华, 浙江 321004, 中国

Recommended Citation:
肖锋,颉志刚,庄建洲. 高温刺激对蟾蜍免疫活性和能量消耗的影响[J]. 生态学报,2015-01-01,35(9):159-166
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