In order to analyze the variations of glacier in Anye Maqen Mountain, RS and GIS technology were used to extract the boundary. Landsat TM and ETM+ images of the years of 1992,2001,2010 were chosen and used. Combining mean annual precipitation and temperature data over the period from 1960 to 2010 from three meteorological stations near the study area, the research about the relationship between the variation of glacier and climate change over the past 20 years was conducted. The results suggest that A'nye Maqen Mountain glacier cover was at the dynamic change status. However, the glacier cover showed a overall trend of retreat over the past 20 years. Glacier cover reduced from 109. 21 km~2 to 99. 34 km~2 during the period from 1992 to 2010 by 9. 04% at a rate of 0. 71 km~2 per year. During 20012010 glacier cover had a retreat rate of 0.38 km~2 per year, which indicated that the glacier retreat rate decreased. Meteorological data shows that there is an obvious increase of temperature, but the precipitation slightly increase, revealing that temperature increase is the vital cause of glacier retreat in Anye Maqen Mountain.