Because of the double impact of climate change and human activities, the hydrological sequence changed, so that the inconsistency directly impacted the representativeness of the hydrological data and the hydrological model parameters and affected the hydrological modeling accuracy and reliability. This paper selected two typical watersheds in the Yellow River Basin and Huaihe River Basin respectively, using the cluster method and Mann-Kendall test method for the hydrological sequence burst detection. It is found that the hydrological sequence data of the Heishiguan Station in the Yellow River Basin changed greatly after 1969,but the hydrological sequence data of the Xixian Station in the Huaihe River Basin had no obvious change. This result indicates that the hydrological sequence of the Heishiguan Station was greatly affected by the climate change and human activities after 1969, and the consistency of the hydrological sequence was destroyed in some extent. In contrast, the Xixian Station was less affected by climate change and human activities, and the stability of the hydrological sequence is better.