The Hailuogou glacier shrinks quickly with climate warming, forming bare and unique soil environment. The bare ground develops into different ecosystems with the succession of time, and provides special environments for scientific research. In this paper, we studied the soil microbial biomass content along the glacial retreat sites of the Hailuogou Glacier with Fumigation extraction method, and then analyzed the relationship between soil microbial biomass and soil nitrogen. We had the following results: 1) Soil NH_4~+-N (P = 0.006), NO_3~-+ NO_2~--N (P = 0.000), total nitrogen (P = 0.000) and soil microbial biomass carbon and nitrogen (P = 0.000, P = 0.000) varied significantly along the glacier succession time, probably due to the different re turn plant residue, decomposition rate, as well as temperature and moisture in different succession sites of different time. 2) The NH_4~+-N content had a range of 4.78 -145.53 mg kg~(-1), NO_3~-+ NO_2~--N of 1.23 -46.08 mg kg~(-1), the total nitrogen of 284.38 -1 980.56 mg kg~(-1), soil microbial biomass carbon 2.76 -1 230.57 mg kg~(-1), and soil microbial biomass nitrogen 0.16 -245.30 mg kg~(-1). 3) The ratio of soil microbial biomass carbon to soil microbial biomass nitrogen ranged from 0.36 to 22.99, mostly less than 6.0, indicating that b acteria dominated the soil microbial community in our study sites at the glacier retreat sites of the Hailuogou Glacier. 4) All soil property indexes except the to tal nitrogen were significantly and positively related to soil microbial biomass carbon and nitrogen (P < 0.01). This research provides basic data support for soil quality assessment in Hailuogou Glacier by indicating the good quality of soil at the glacier retreat sites.