The responses of tree ring width of Pinus massoniana at different latitudes (26°33'N: northetn part, 25°47'N: center part, 24°42'N: southern part) to climate changes in Nanling Mountains of south China were investigated by choosing P. massoniana sample trees and sampling sites. Samples were collected at the 1.3 meter height of sample trees and then dried, polished and measured ring wheel width by using LinTab5 type ring wheel measuring analysis system, according to the normal process of tree-ring analysis, the chronology was attained using the software ARSTAN to acquire the relationships between tree ring widths and climatic variables including temperature and precipitation after measuring and cross-dating these samples by COFECHA program and LINTAB program. Through correlation analysis, the responses of P. massoniana to the climate changes were studied. P. massoniana tree samples at three different latitudes in Nanling Mountains were used to build the chronology tree-ring width at different latitudes, explored the responses of tree growth to climate changes at different latitudes in 1951~2010, and then conducted a correlation analysis between climate factor and tree-ring width. The tree-ring width of the wood sample from the northern region was mainly influenced by the temperature of winter and summer. The rising temperatures of winter and summer promoted the radial growth of P. massoniana. Also in the central latitude region, the rising temperatures of winter promoted the radial growth, while the rising temperatures of summer, to some extent, inhibited the radial growth. The tree-ring widths from the southern region were mainly influenced by the climate of summer. The rising temperatures of summer inhibited the radial growth of P. massoniana. The radial growth was significantly associated with precipitation of the southern region. The radial growth of the central latitude region was related with precipitation in October of last year, but not significant.