The responses of N_2O emission fluxes to soil temperature and moisture of Kobresia humilis meadow were studied in Haibei Station on Qinghai-Tibetan Plateau with culture method in laboratory. It was revealed that the flux decreased gradually with the increase of temperature during the initiate culture phase,and the highest rate was (4.75 0.24) g/(kg·h). The lowest emission rate was (2.92 0.19) g/(kg·h). After 7 days culture,the N_2O flux decreased and became N_2O sink,and then increased. The highest flux was (0.67 0.06) g/(kg·h) at 30℃,but all plots were significant lower than initiate culture (P<0.05). Along with the increase of soil moisture,the flux of initiate culture showed a high-low pattern.The lowest value was (3.38 0.25) g·(kg·h) at 75% and the highest was (3.620.38) g/(kg·h) at 60%. Along with the increase of cluture time,the emission rate decreased gradually,and the highest value was (0.55 0.32) g/(kg·h).The highest emission rates for 1 day and 7 days culture were reached at 45% soil moisture and 20℃,and 60% soil moisture at 30℃ separately. However,these value dropped to the lowest at 45% soil moisture and 30℃,and 75% soil moisture and 30℃. This indicated that the soil N_2O emission would decrease under climate warming and precipitation increasing conditions in the future.