globalchange  > 气候变化与战略
CSCD记录号: CSCD:5836096
论文题名:
PEG胁迫下油菜种子萌发期生理及基因表达研究
其他题名: Physiological and Antioxidant Enzyme Gene Expression Analysis of Brassica napus during Seed Germination Stage under PEG Stress
作者: 方玉洁; 李剑; 夏凌君; 孙念礼; 王幼平
刊名: 分子植物育种
ISSN: 1672-416X
出版年: 2016
卷: 14, 期:10, 页码:2069-2074
语种: 中文
中文关键词: 甘蓝型油菜 ; 白芥 ; 杂种后代 ; 干旱胁迫 ; 抗氧化酶基因
英文关键词: Brassica napus ; Sinapis alba ; Hybrid progeny ; Drought stress ; Antioxidant enzyme gene
WOS学科分类: AGRONOMY
WOS研究方向: Agriculture
中文摘要: 油菜是我国重要的油料作物之一,油菜生产对我国食用油供给安全和国民经济发展有着举足轻重的影响。随着全球气候剧变,干旱胁迫已成为制约我国农业发展的重要因素之一,给油菜生产带来严重损失。因此,研究甘蓝型油菜干旱应答的机制,提高甘蓝型油菜的抗旱性有着十分重要的意义。本研究对甘蓝型油菜与白芥种间杂交的3个后代株系和扬油6号(Y6)种子萌发期的抗旱性进行了鉴定,研究了15% PEG(polyethylene glycol)模拟干旱胁迫条件下4个株系的形态变化、生理生化指标以及抗氧化酶系统基因表达情况,发现在15% PEG模拟干旱胁迫的条件下,与对照(Y6)相比,杂种后代株系种子萌发期的根长及苗高受抑制程度弱于对照,丙二醛(malondialdehyde, MDA)含量显著高于对照,部分后代株系的活性氧类物质积累显著低于对照,部分抗氧化酶基因在杂种后代中的表达量显著高于在Y6中的表达量,说明杂种后代可能从亲本白芥中继承了抗旱性较强的特点。
英文摘要: Brassica napus is one of the most important oil crops in China. Rapeseed production has a pivotal effect on the edible oil supply security and the national economy in our country. With the global climate change, drought has become a main restriction factor for agricultural development, and brought heavy losses to rapeseed production. Therefore, investigating the mechanism of rape response to drought stress and improving the drought resistance of rapeseed possesses great significance. In this study, the drought resistance of three offspring lines of intergeneric somatic hybrids between Brassica napus and Sinapis alba and B. napus cv. Y6 (Yangyou 6) during seed germination stage were evaluated. The morphological, physiological, biochemical changes and the transcript abundance of a series of antioxidant enzyme encoding genes were examined. Under drought stress simulated by 15% PEG (polyethylene glycol) treatment, compared with the control (Y6), the root and shoot development of the hybrid offspring materials were less inhibited. The MDA (malondialdehyde) content of the hybrid offspring materials was lower than that of Y6, and the ROS (reactive oxygen species) accumulation in the hybrid offspring materials was higher than that in Y6. Moreover, the transcript level of some antioxidant enzyme encoding genes was obviously higher in the hybrid offspring materials than that in Y6. Our results indicated that the hybrid offspring materials may derive stronger drought resistance from the parent-Sinapis alba.
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/150779
Appears in Collections:气候变化与战略

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作者单位: 扬州大学生物科学与技术学院, 扬州, 江苏 225009, 中国

Recommended Citation:
方玉洁,李剑,夏凌君,等. PEG胁迫下油菜种子萌发期生理及基因表达研究[J]. 分子植物育种,2016-01-01,14(10):2069-2074
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