The small scale temperature enhancement experiment was simulated with open-top chamber (OTC) in two years,and the response of temperature enhancement on growth and root soil respiration rate and enzyme activity of spring wheat was studied, so as to reveal the spring wheat response mechanism to global climate change and provide scientific basis for the spring wheat cultivation with high yield and good quality under the background of global climate change. The results showed that at the mature stage, the contents of chlorophyll a,b,carbohydrate,protein,crude protein,crude fat,crude fiber,crude ash,nitrogen free extract,and the plant height, stem diameter, leaf area index, specific leaf weight,grains per ear and 1 000-seed weight presented OTC > CK(without temperature enhancement). The simulated temperature enhancement increased the soil respiration rate and soil enzyme activities at the growth stage of spring wheat. There were significant exponential relationship between soil respiration rate and enzyme activities and soil temperature. To sum up,enhancement of temperature was beneficial to improve the growth and root soil respiration rate and enzyme activity of spring wheat.