Livestock manure is an important agricultural source of greenhouse gas (GHG) emission. Proper management of livestock manure can mitigate GHG emission and reduce environmental pollution. To identify the effectiveness of appropriate manure management in mitigating GHG emission,the livestock manure from different breeding modes in Hubei Province were taken as a case study. The optimal manure management modes were selected according to the manure characteristics of different livestock,natural conditions and breeding modes. Using the method proposed by Intergovernmental Panel on Climate Change,the GHG emission factors for livestock manure were estimated,and the mitigation potential of GHG emission in livestock manure with adapted optimal management were evaluated. The results showed that,with optimal management mode,the mitigation potential of GHG emission ranged from 1.98 to 357.82 kg(CO_2 -eq)·head~(-1)·a~(-1) . Given the development trend of the livestock in Hubei Province,GHG emission could be reduced to 3227.8*10~3 t CO_2 -eq in 2020 with optimal manure management. The mitigation potential of regional GHG emission was closely related to the breeding scale,structure and mode and the manure management. The different characteristics of livestock manure brought about the various reduction efficiency of CH_4 and N_2 O. The key measure to reduce the regional GHG emission was to optimize the mature management for scale-feed livestock. Therefore,integration of the appropriate livestock manure management with regional natural conditions and breeding characteristics was an effective means to mitigate regional the GHG emission.