The stress effect is a characteristic of Dao-di herbs caused by environmental gene expression of medical plants is influenced by environmental changes and finally affects the formation and accumulation of metabolites. Using Scutellaria baicalensis as material, active component of wild type of S. baicalensis from 19 production areas were analysed; It was found that climate change can influence the accumulation of active components. Then, S. baicalensis suspension cells was exposed to various environments, and enzyme activity and gene expression were measured, indicating the molecular mechanism of stress effect on S. baicalensis. Hence, we found the prerequisite and method to study the stress effect on Dao-di herbs, and we hope this research can provides some references for another studies of Dao-di herbs.