The Qinghai Lake watershed has mostly alpine meadow cover. We used a modified Costanza's model,revised to incorporate the extent of vegetation lushness and the effective time of vegetation providing ecosystem services to evaluate ecosystem service values(ESV)and analyze spatial patterns for the year 2000 and 2012. The results show that the total ESV of the Qinghai Lake watershed was 1.54*10~(12) CNY,a rise of 1.20*10~8 CNY on 2000. Water was the main source of Qinghai Lake watershed ESV,followed by grassland,woodland,farmland,and desert in 2000; however,the total ESV of desert exceeded farmland because of farmland area decline and desert area increases in 2012. Different land cover type spatial patterns in the whole space determined spatial pattern heterogeneity for ESVs in the Qinghai Lake watershed. Grassland ESV showed a latitudinal zonal pattern across the whole space,while some areas showed vertical zonal variation because of vegetation coverage and growing season changes across latitudes and vertical zones. ESV spatial patterns change significantly under the double disturbance of human activities and climate change,some regions with high ESVs from concentration distribution to uniform, grassland ESV in the space increased,and the range of Qinghai Lake with high ESV expanded. Land use change under human disturbance was the main reason to reduce total ESV,and the potential impact of climate change on ecosystems helps to increase ESV of the watershed. These data provide a scientific basis for the government to make ecological environmental protection policy under climate change for the Qinghai Lake watershed.