The MIS 3 (Marine Isotope Stage 3) was a special period during the past global climate evolution process. It is important for getting more details about MIS 3 for deeply understanding the regional change of paleo-climate and paleo-environment. The paleo-Daocheng Ice Cap is situated in the southeastern Tibetan Plateau, where the glacial remnants (including the oldest moraines in the Hengduan Mountains) have been well preserved, which provides a natural laboratory to study the glacial history and climate change during Quaternary. Although there has been a lot of work in this area,consensus has not reached on the time of the maximum glaciation and whether glacier advance during MIS 3. In this paper,the possibility of MIS 3 glacial advance in paleo- Daocheng Ice Cap is explored based on the climatic records (stalagmites,ice cores,marine sediments and pollen data) and the dating data of glacial advance in the plateau (include the paleo-Daocheng Ice Cap) and around areas during MIS 3. The results showed that glaciers had advanced during 43 ~ 53 ka (MIS 3) . This research will provide new data for climate and environment reconstruction.