The N_2O emission fluxes from grassland ecosystem and the relationship between N_2O emission fluxes and precipitation and air temperature were studied,using static chamber technique and gas chromatography,in Haibei Station of Chinese Academy of Sciences during consecutive 2 years: 2013 and 2014.The results revealed that the annual mean fluxes of N_2O emission rate were around 32.43.1mug/(m~2·h).The emission rate 41.14.3 mug/(m~2·h) during the growing seasons was significantly higher than that 20.23.2 mug/(m~2·h) during the non-growing season.The significant differences of N_2O emission fluxes were found between different sampling days.The N_2O emission fluxes in an alpine meadow increased with daily air temperature increasing.The relationship between fluxes of N_2O emission and precipitation was negative.The N_2O emission fluxes will increase with global warming in the alpine meadow in future.