This article achieved the extraction of land use and land cover change information of the Daling River Basin from 1986 to 2014 (7 periods) based on Landsat remote sensing image. It was found that land use change has a negative effect on climate by analyzing climate change (13 periods). The results showed that: 1) Construction land and farming-forest land expanded obviously,the area increased respectively by 322.30 km~2 and 1 504.94 km~2 accompanying by waters and beach and other unused land shrunk significantly,the area decreased respectively by 102.42 km~2 and 1 724.61 km~2; 2) Land use of Daling River Basin lead to average annual rainfall,average relative humidity and average wind speed minor decreasing,decreased respectively by 14.94 mm,0.2% and 0.04 m/s; average temperature rising slowly,increased by 0.1℃; 3) Policies of returning farmland to forest and grassland and establishing basin reserve management can increase vegetation and water coverage area,thus relieve temperature rise brought by urban heat island effect and improve the quality of ecological environment in the Daling River Basin.