In this study, Hoh Xil area, the hinterland of the Tibetan Plateau, was taken as the research area. The climate evolution in Hoh Xil since the last interglacial age was reconstrcted by analyzing colour, magnetic susceptibility, total organic carbon (TOC),and grain size of lacustrine sediments. Compared with Marine Isotope Stages (MIS), there was both a good agreement with climate evolution from other areas in the world and unique local characteristics. Similar to MIS5, climate change during the last interglacial age can be classified into five sub-stages, but the phases were not an exact match with each other. According to our results, the warmest period occurred during MIS5a instead of MIS5e. Overall, there was no warm and humid climate and environment during the last glacial age unless the interstage of the last glaciation. In this period, low TOC content and high peaks of colour and magnetic susceptibility indicated that lake level rose slightly. So it possibly corresponded to the periods of MIS3c and 3a with warm and dry climate. Perhaps due to shrinkage of the water body and increased hydrodynamic force as a result of the strong tectonic movement, the deposition conditions deteriorated and TOC content decreased. Low values of proxies during other periods indicated a cold climate. In these periods, the occurrence of some peaks of high values was consistent with the Dansgaard-Oeschger cycle. High values of climate indicators could be attributed to the warmer Holocene since 10000 years ago.