Based on the observed data from 17 meteorological stations and 2 hydrometric stations in the Heihe River Basin and its peripheral areas in recent 50 years,a variety of methods, such as Mann-Kendall (M-K),Pettitt, Yamamoto and Accumulative departure curve,were used to explore the spatiotemporal variation of hydro-meteorological variables. Results showed that the spatial distribution of hydro-meteorological variables in the Heihe River Basin was sensitive to topography. The magnitude of mean air temperature,minimum air temperature,maximum air temperature, sunshine duration and mean wind speed increased,but precipitation and mean relative humidity decreased from south to north. Precipitation,mean air temperature,minimum air temperature,maximum air temperature and runoff volume at Yingluoxia Hydrometric Station in the Heihe River basin exhibited the significant increase trends,while the mean wind speed and sunshine duration showed the remarkable decrease trends. Runoff volume at Zhengyixia Hydrometric Station had a slight decrease trend,and there was no obvious trend in mean relative humidity. Abrupt changes of annual mean air temperature,annual maximum and minimum air temperature were detected in the 1990s. The abrupt changes of precipitation and runoff volume at Yingluoxia Hydrometric Station occurred in 2004 based on the cross-validation, implying that different detection methods should be used to increase the credibility and certainty of the change points.