[Objective]To study the seasonal variation of soil enzyme activities and its response to environmental factors and to understand the effect of microbial factor in the regulation of soil carbon cycle in the context of climate change. [Method]The infrared radiation heater and the 96 micro-plate method were used to assay the activity of the hydrolytic and oxidative enzymes of the soil,and determine the role of environmental factors in addressing the seasonal variation in the activity of soil enzymes. [Result]During growing season,the warming treatment significantly increased the soil temperature by 1.91℃,leading to a 12.15% decrease of organic carbon content and significant increase of 40.30% and 61.29% of microbial biomass carbon (MBC) and nitrogen (MBN). During nongrowing season,however,although the warming treatment increased the soil temperature by 2.24℃,no significant effect was found on MBC or MBN. The effects of warming treatment on specific enzyme varied in different seasons. During growing season,the activities of specific enzyme in warming treatment were lower than those in control except for NAG,while they were higher during non-growing season except for BG and LAP. [Conclusion]The environmental factors accounted for 82.5% of the total variations of soil enzyme activities,while the activities of specific enzyme were significantly affected by NH_4-N and MBN. The other environmental factors (soil moisture,MBC, DOC,MBC /MBN and NO_3-N) were the main reasons for the variations of specific enzyme activities in different treatments and seasons.