This study aimed to better understand the influence of global climate change on soil carbonyl sulfide(COS). Dynamic chamber and gas chromatography-mass spectrometry methods were used to investigate the effects of various environmental factors(i.e. temperature, soil water content and carbon dioxide(CO_2)concentrations)on soil COS fluxes under laboratory conditions. Soil cores were collected from typical sugarcane fields in south China. Parts of the soil cores were collected without disturbance(i.e., intact soil cores), whereas the remaining were broken and mixed after passing through an 8-mm mesh sieve(i.e., disturbed soil cores). The results showed that COS fluxes from the intact soils were significantly different from those of the disturbed soils. The COS emission rates from intact soils were higher than those from the disturbed soils, whereas the COS uptake rates showed an inverse pattern. COS uptake rates were reduced remarkably after sterilization, indicating that they were mainly controlled by biological processes. Temperature significantly influenced the COS fluxes of disturbed soils, and the lowest COS uptake rate(100.4 pmol·m~(-2)·s~(-1))occurred at 25 ℃. Soil water content significantly affected the COS fluxes in intact soils, and the lowest COS uptake rate(0.9 pmol·m~(-2)·s~(-1))occurred at 50% water holding capacity. High ambient CO_2 concentration(8520 mg·m~(-3))inhibited COS emission from intact soils. The results suggested that the effects of soil structure, temperature, humidity, and ambient CO_2 concentration should be considered when estimating the global soil COS fluxes.