The alpine meadow, a main type of grassland on Tibetan Plateau, playing a key role in regulating greenhouse gases(GHGs) flux. To date, it remains unclear how GHGs flux of the alpine meadow responses to warming, especially to multi-level warming. Simulated multi-level warming experiment, therefore, improves the prediction of GHGs flux dynamics. For further understanding the effects of climate warming on GHGs flux in the alpine meadow, we hypothesized that the major GHGs flux, including ecosystem soil CO_2 respiration, CH_4 consumption and N_2O emissions all might be enhanced under warming, we then conducted a simulated gradient warming experiment (T0, no warming; T1~T4 represent the temperature increased by 1, 2, 3 and 4 ℃, respectively) and GHGs flux measurements in an alpine meadow in Namtso of Tibetan Plateau, using open-top chambers(OTCs) and static chamber method, respectively, for the following there growing seasons from year 2013 to 2015. The observations showed that: (1) When compared to the control plots, the three year averaged below ground 5 cm soil temperature increased 1.73 ℃(T1), 1.83 ℃ (T2), 3.03 ℃ (T3) and 3.53 ℃ (T4), respectively for the four warming treatments; (2) The averaged flux of CO_2, CH_4 and N_2O for growing season in alpine meadow were (42.69.11) mg·m~(-2)·h~(-1), (-7.968.76) mug·m~(-2)·h~(-1) and (0.30.46) mug·m~(-2)·h~(-1), respectively; And (3) the GHGs flux were regulated by both soil temperature and moisture, leading to a non-significant response to warming because of the relative variability in soil moisture. Our results suggested that alpine meadow can keep its GHGs flux stable to warming in short term by internal modification (e.g. uncertainty in soil moisture variability accompanied with various warming conditions).