With the growth of economics population, the aquaculture industry in coastal areas developed rapidly. Therefore, the influence of CO_2, CH_4 and N_2O fluxes from aquaculture areas can not be ignored. In this study, variations of CO_2,CH_4 and N_2O fluxes at water-air interface in the daytime were determined using a floating chamber- gas chromatography method in shrimp pond from June 2013 to April 2014 in the Yellow River estuary. Meanwhile, some of environmental factors were also measured. The results showed that the ranges of CO_2,CH_4 and N_2O fluxes were -71.140 3~7.278 6, -0.005 8~0.016 5, -0.002 0~0.007 9 mg·m~(-2)·h~(-1), and the mean values were -36.347 5,0.005 9, 0.002 7 mg·m~(-2)·h~(-1), respectively, indicating that the culture pond acted as an emission of CH_4 and N_2O and consumption of CO_2. Seasonal variations of greenhouse gases from culture pond were observed, the fluxes of CH_4 and N_2O were large at high temperature. Correlation analysis results showed that temperature, salinity, SO_4~(2-), Cl~- concentration and phosphorus had a certain influence on greenhouse gases fluxes, and the correlation between salinity and N_2O flux, and SO_4~(2-) and CO_2 flux reached significant level. The greenhouse gas fluxes of the Yellow River delta aquaculture pond were different from that of natural water and other aquaculture ponds, which might due to geographical differences, breeding way, use of fungicides and so on. In addition, culture pond was seriously affected by anthropogenic activities, and the greenhouse gases emission were complicated and might be controlled by the interactions of multiple controlling factors, which needed further research. The results of this study have certain significance for the comprehensive evaluation of the impacts of aquaculture influence on regional climate change, and deepening the impact of human activities on global warming.