In order to effectively protect rare and endangered plants, botanical gardens have moved from ex situ conservation to the combination of ex situ conservation and reintroduction. Its living collections, knowledge, skills, and facilities can be used in the process of plant reintroduction. Early plant reintroduction practices have focused on increasing the survival rate of seedlings or seeds by means of horticulture, and later emphasized population recovery and plant reintroduction in the context of ecosystem restoration. In recent years, emphasis has been placed on plant reintroduction in the context of global change. Nowadays, plant reintroduction research and practice at botanical gardens is mainly focused on factors affecting reintroduced individuals, genetic diversity associated with reintroduction, the impacts of global change on reintroduction, and the criteria for successful reintroduction. With human disturbance and global change, botanical gardens need to take into account integrating in situ conservation, ex situ conservation, and reintroduction to effectively protect plant diversity.