Based on the data of daily temperature and precipitation of six meteorological stations in Ningjiang River Basin and hydrological data gathered from Hekou station from 1953 to 2013,landscape pattern metrics with land use and land cover date derived from the Landsat TM imagery acquired in 1986,1995,2005 and 2013,we used the methods of Mann-Kendall trend analysis,Wavelet analysis and SCRAQ method aiming to analyze the contribution ratio of precipitation and human activities to the runoff in the Ningjiang River Basin. The results showed that:(1)the temperature presented significantly increasing trend and runoff demonstrated the decreasing trend,the precipitation appeared weakly increasing trend;(2)there was obvious correlation coefficients among temperature,precipitation and runoff at the scale of 27-year before 1990,while the scale was destroyed by human activities after 1990,it was still synchronization in scale of 11-year between runoff and precipitation,indicating that runoff changes were mainly affected by the precipitation;(3)with the development of economy and soil and water conservation from 1980s,woodland area increased by 26.2%, grassland area land reduced by 41.6% and cultivated area reduced by 61.2%.The large area of land use change occurred in the northern Xingning;(4)the period from 1953 to 1958 was taken as the base period, the contribution rates of the precipitation and human activities to the increased runoff were 62.2% and 37.8% in the period from 1959 to 2008,the rates to the decreased runoff were 45.8% and 54.2%,respectively, in the period from 2009 to 2013.