Taking the upper reaches of Huaihe River Basin as a case study,we investigated the variation of runoff in order to provide the scientific basis for flood control,water resource reasonably planning and economy sustainable development.Based on daily runoff data from 1975 to 2014in two hydrological stations(Dapoling and Changtaiguan)of Huaihe River,Gini coefficient and Lorenz asymmetry coefficient in two hydrological stations were statistically analyzed after building the Gini coefficient runoff uneven distribution model,annual runoff.The characteristics of annual runoff change were analyzed by using the linear regression,Mann- Kendall(MK)test,rescaled range(R/S)analysis,wavelet transforms and other mathematic statistic methods. Some important results were obtained as follows.(1)The runoff trends of Dapoling and Changtaiguan hydrological stations were weak decrease with time at rates of-1.68m~3/(s·decade)and -3.83m~3/(s·decade), respectively.Gini coefficient had a non-significant change.The average Gini coefficients of two stations were 0.54and 0.53,respectively,indicating that the annual distribution of runoff was heterogeneous.The Lorenz asymmetry coefficients(S>1)of two stations accounted for 65%and 57.5%,respectively,indicating that it had greater percentage of the runoff in large runoff months.Lorenz asymmetry coefficient of annual runoff showed insignificant increasing trend,showing that the inhomogeneous degree of the runoff distribution had weak increase.(2)Hurst indexes of the two hydrological stations were slightly great than 0.5in annual mean runoff by R/S analysis,indicating that there was obvious Hurst phenomenon,and showing that the slight decreasing trend in upper reaches of the Huaihe River would continue in the near future.(3)The oscillating periods of runoff in the past 40years had complex variation characters with a nested structure on multiple time scales.The wavelet analysis showed that there were three periodic oscillations of 2~5years,10~15 years and 25~30years.After the analysis of wavelet variance plots,it was found that there were 3years, 12years and 28years periodic fluctuations of annual mean runoff.The two stations might have a quasi-period of 28years,which need a longer time series to prove.The response of river water resource to both human activities and climate change,as the theoretical basis to understand the river hydrological process and the sustainable use of regional water resources,has gained more and more attentions on water research during the last decades.