Wheat is the most widely cultivated cereal crop in the world. But wheat production is being seriously affected by biotic and environmental stresses such as diseases,drought, salt,and high temperature with the global climate change. It is high necessary to improve wheat for biotic and abiotic resistance by genetic engineering technique. Compared with other major crops,wheat modification by transgenic approach is lagging behind in the past two decades. Transformation protocol and genotypes will all influence the development of genetic modified wheat varieties. Screening wheat genotypes with high plant regeneration potential from new released commercial wheat varieties or lines will be great beneficial to the improvement of this crop by genetic engineering strategy. Immature grains of eight new wheat cultivars (Zhengmai 0896,Zhengmai 1354,Zhengmai 1836,Zhengmai 1860,Zhengmai 5135, Zhengmai 7698,Zhengmai 9023,and Zhongyu 1439) released in Henan province recently collected in growth chamber post anthesis for 15-16 days,and their immature embryos of 1.5-2.0 mm in size were isolated and cultured on callus induction medium containing three auxins 2,4 -D,dicamba and picloram respectively,after carefully removing the embryonic axis. By investigating total calli,differentiated calli,and regeneration shoots during the tissue culture course,differentiated calli rate and regenerate rate were calculated for each treatment of each wheat variety. The effect of the three auxins 2,4 -D,dicamba and picloram on plant regeneration of their larger immature embryos was evaluated,and the regeneration abilities among the varieties were compared. Adding dicamba in callus induction medium can greatly increase the seedling regeneration frequency. Plant regeneration efficiency of all the tested wheat varieties was more than 189%,among which the regeneration efficiency of six varieties such as Zhengmai 1836 and Zhongyu 1439 was over 400%. When picloram was applied in callus initiation medium,plant regeneration efficiency of the eight wheat varieties was more than 210%,among which the regeneration efficiency of four varieties such as Zhengmai 1860 and Zhengmai 5135 was over 1100%. In the condition of 2,4 -D used on callus initiation medium, Zhongyu 1439 showed the highest regeneration ability followed by Zhengmai 5135 and Zhengmai 1354,while a regeneration efficiency less than 60% was obtained in Zhengmai 1860 and Zhengmai1836,and no regeneration shoot was obtained in Zhengmai 7698,Zhengmai 0856 and Zhengmai 9023. Results indicated the optimal auxin for the plant regeneration of larger immature wheat embryos was dicamba followed by picloram,but difference related to genotypes was also detected. In the tested eight wheat varieties, the order of the first four ones on the regeneration potential of immature embryos was Zhongyu 1439,Zhengmai 5135,Zhengmai 5135 and Zhengmai 1354. Thereby, the four new wheat varieties evaluated in this study with high regeneration potential of immature embryos are suggested to be used in wheat transformation or transgenic breeding.