Tree-ring data of six dominant tree species (Platycarya strobilaceaisthe repeated tree species) of two typical karst areas and a non-karst area in Guizhou Province, China were used to develop six tree-ring chronologies. The different and similar responses of radial growth to climate change during the past five decades of different tree species of different areas were investigated, and the effects of climate factors to the six tree species were also discussed. Results show that the water-heat condition before the monsoon season is the main climate limitation for the radial growth of both coniferous and broad-leaved trees in karst forests. The reduction of monthly mean precipitation and the rise of monthly mean temperature before the monsoon season lead to faster evaporation of soil moisture before the monsoon, thus, water stress is enhanced, resulting in drought stress on the radial growth of both coniferous and broad-leaved trees in karst forests. The Palmer drought severity index (PDSI) indicates weak restrictions on most of the tree growth, but in fact, water leakage aggravates the water stress for the growth of coniferous and broad-leaved trees in the karst areas. Precipitation is not the limitation for trees growing in the non-karst area, because lower temperature at higher elevation of sampling point in the non-karst area reduces the evaporation of soil moisture from last winter to next spring. The soil and land form in non-karst area is crucial for water reservation, meanwhile, high elevation also changes the precipitation patterns before the monsoon, which eliminates the water restriction of soil moisture for the growth of trees before the monsoon. The low temperature in previous December is the main limitation for the radial growth of coniferous trees with a positive response signal, and broad-leaved trees are slightly promoted by temperature and precipitation in growing season.