The North Atlantic plays an important role in the global climate system and its link with climate change has received much attention. Marine Isotope Stage(MIS) 5e with a warmer climate than the Holocene is considered as an analogy for future warming climate. To date, there are few deepwater temperature and salinity comparisons between these two recent interglacial periods in the North Atlantic. Benthic foraminifera oxygen isotope (delta~(18)O) has long been analysed in paleoclimate studies since its variation closely related to global ice volume,temperature and ocean circulation changes. In this study, a comparison of benthic foraminifera oxygen isotope during the Holocene (0~12ka)and MIS 5e(116~130 ka) stages from 47 deep-sea sediment cores in the North Atlantic is presented to investigate whether a systematic delta~(18)O difference exists during the last two interglacial periods. This database suggests the delta~(18)O signatures during the MIS 5e were systematically lighter(ca. 0.08) than those in the Holocene, indicating potential reduced global continent ice volume and/or warmer deep-sea temperature in the North Atlantic. Although the delta~(18)O differences between Holocene and MIS 5e are small, the systematical trends in our database confirmed by t-test provide confidence in our proposal that the different climate signals are observed by the delta~(18)O comparison. This study further examines the contribution of sea level or temperature changes in the MIS 5e and Holocene delta~(18)O differences tentatively based on the empirical relationships between delta~(18)O and sea level/temperature in the palaeoceanography field,which suggests a dominant control of higher sea level and a slight warmer North Atlantic deepwater in the MIS 5e. A further regional examination of delta~(18)O difference between Holocene and MIS 5e shows larger temporal variations in the intermediate depth cores between 1 and 2 km water depths (>0.2%o) than cores below,implying enlarged magnitude of temperature changes, and the heavier delta~(18)O of cores above 1.5 km during the MIS 5e averaged by ca.O. 36%o might be associated with regional ocean circulation changes. Moreover, the decrease of ca.O. 12%o in MIS 5e benthic foraminifera delta~(18)O of high latitude deep cores(>450N) is more pronounced than that of low-middle latitude deep cores(0° ~45°N),inferring a more significant warming in the high latitude deep water, which is in accordance with sea surface temperature reconstructions and modelling results. Benthic oxygen isotope investigation based on multiple sediment cores is a useful approach to identify regional changes in oceanographic changes. Changes in regions such as the high latitude and intermediate depth North Atlantic would deserve special attention in future climate simulations.