The extreme low temperature stress caused by climate change severely affects plant growth and development,hence leading to significant grain yield loss in wheat. Our previous studies demonstrated that the stress priming at the early growing stage induces cold tolerance in wheat,which is closely related to the abscisic acid (ABA) signaling pathway. However,the priming strategy is limited in wheat production,due to the short-lasting effects of drought priming. A novel ABA-mimicking ligand with fluorine atoms,named AMFs,possesses higher photo-stabilization,long-lasting effects and low-cost advantages,in relation to ABA. Hence,understanding the mechanism of ABA, AMF and priming-induced cold tolerance will be helpful to reveal the mechanisms of induction of cold tolerance in wheat by chemical approaches and priming strategy,and support the establishment of optimal cultivation techniques for enhanced cold tolerance in wheat.