Due to the carbonate nature, the critical zone in karst region is very complex, and characterized by thin soil layer with low water holding capacity, lot of cracks with high percolation, which result in a series of ecological environment problems, such as frequent water deficit, fragile ecosystem and rapid pollutant migration. Therefore, knowledge of ecohydrological processes in karst region is essential for ecosystem protection and rational utilization of karst area. To date, many progresses in karst ecohydrology have been obtained. These results improve our insight in evapotranspiration and water sources of karst plants, hydrological processes on karst slopes, ecohydrological effects of environment changes and etc. and provide theoretical and technical supports for ecological restoration, environment protection and water resources utilization. However, owing to the extreme complex critical zone, there still exist many shortcomings and uncertainties of these results. Focusing on ecohydrological processes, karst catchment-scale ecohydrology, and karst ecohydrological models, this paper reviewed the main progresses in karstic ecohydrology, introduced our main achievements in the corresponding areas, and pointed out the points that need to be emphasized.