Atmospheric nitrogen deposition is one of the focus issue of global change, and this study was conducted in a subtropical evergreen broad-leaved forest in Mount Wuyi aiming at measuring soil respiration and the effects of nitrogen deposition on soil CO_2 emission. Nitrogen addition experiments were carried out within the forest selected for 12 months by Li-6400. Four N addition treatments (in three replicates) were established in mature forest: control, No N (N0, 0 kg·hm~(-2)·a~(-1)), low N (N1, 50 kg·hm~(-2)·a~(-1)), medium N (N2, 100 kg·hm~(-2)·a~(-1)) and High N (N3, 150 kg·hm~(-2)·a~(-1)). Meanwhile, we measured temperature and soil moisture to explore the response of soil temperature and soil moisture to soil respiration in this region. The results showed that, (1) Soil respiration in a subtropical evergreen broad-leaved forest in Mount Wuyi had the obvious seasonal dynamics and there was a lower soil respiration in January and higher soil respiration in August. (2) There was an obvious seasonal dynamics in soil respiration, and soil respiration showed the unimodal curve with the peak in August. Repeated measurement of variance results showed that there was no significant effect of nitrogen deposition on soil respiration (P>0.05). (3) There was a significantly exponential relationship between total soil respiration and soil temperature, while N1, N3 increased Q_(10) and N2 decreased Q_(10), and the Q10 in N0, N1, N2, N3 was 1.52, 1.57, 1.44 and 1.56, respectively. There was a significantly quadratic curve between total soil respiration and soil water content in 0~5 cm and 5~10 cm, and R~2 was 0.156~0.354, 0.239~0.387, which was higher than the correlation coefficient between total soil respiration and soil temperature, indicating that the effect of soil water content on soil respiration was weak than soil temperature. And (4) the total soil respiration carbon emission in N0, N1, N2, N3 was 5.67, 5.98, 6.22 and 4.22 t·hm~(-2)·a~(-1), respectively. Lower N promoted soil respiration and higher N inhibit soil respiration. Variance analysis showed that nitrogen deposition had no significant effect on the annual flux of respiration and heterotrophic respiration.