globalchange  > 气候变化事实与影响
CSCD记录号: CSCD:6283873
论文题名:
氧化亚氮还原酶基因nosZII及与环境的关系研究进展
其他题名: Research progress of nitrous oxide reductase gene (nosZII) and its relationship with the environment
作者: 刘春梅1; 魏文学2; 盛荣2; 邢肖毅1; 谌星1
刊名: 应用与环境生物学报
ISSN: 1006-687X
出版年: 2018
卷: 24, 期:3, 页码:722-730
语种: 中文
中文关键词: nosZII基因 ; nosZI基因 ; N2O还原能力 ; 群落结构
英文关键词: N2OR ; nosZII gene ; nosZI gene ; N2OR ; N2O-reducing ability ; community structure
WOS学科分类: GENETICS HEREDITY
WOS研究方向: Genetics & Heredity
中文摘要: 温室效应在全球范围内日趋严重.其中氧化亚氮(N_2O)作为最重要的温室气体之一,增温潜能是二氧化碳(CO_2)的298倍,且其浓度仍呈逐年上升趋势.氧化亚氮还原酶(N_2OR)能将N_2O还原成氮气(N_2),而nosZ基因是编码N_2OR的唯一基因.除了熟知的nosZI基因外,新发现的分支nosZII基因也能编码N_2OR.在国内外研究基础上本文总结nosZII基因的基本概况,分析其与nosZI的主要区别,阐述其相关反应机制,并归纳不同环境因素对nosZII基因表达的影响.研究表明,nosZII主要存在于epsilon-变形细菌、拟杆菌、产水菌中,含nosZII的微生物中有部分种群缺乏nirS和nirK基因,且该类微生物在还原N_2O功能上具有较大潜能.影响含nosZII微生物的丰度和种群结构的环境因子较多,主要包括土壤结构、土壤pH值、土壤C/N、温度、湖泊物理梯度等,其中pH值和C/N可能是主要的影响因素.随着分子生物技术手段的进步,对含nosZII微生物的生态功能的探索已取得了重要进展,但还需进一步深入.今后还需对含nosZII的微生物群落结构特征、微生物丰度和多样性影响因素以及对具有较强还原N_2O能力的含nosZII菌株进行更深入的研究,为降低N_2O排放、有效减控温室气体提供理论依据.
英文摘要: The greenhouse effect has become increasingly serious globally. Nitrous oxide (N_2O) is both the major ozone depleting substance and a potent greenhouse gas having a global warming potential 298 times that of CO_2, and the N_2O concentration is still increasing at an annual rate of about 0.8 *10~(-9). Nitrous oxide reductase (N_2OR) can reduce N_2O to N_2, and until recently, the nosZ gene was the only gene known to be able to encode N_2OR. Besides the well-known nosZI, a new lineage of the N_2O-reductase (nosZ clade II), which is abundant and widespread in soils, has been identified. In this paper, the main characters of nosZII-containing microbial communities and the related working mechanisms are summarized. In addition to the main differences between nosZI and nosZII, the important environmental factors that regulate the composition, abundance, and expression of nosZII-containing communities are also discussed in this paper. Studies have shown that nosZII communities are distributed among a diverse range of bacterial and archaeal phyla, such as Epsilon-proteobacteria, Bacteroidetes, and Aquificae. Interestingly, most of the nosZII microbes lack a nitrite reductase encoding gene (nirS or nirK) and are therefore unable to denitrify, indicating the importance of these communities as N_2O sinks. Soil properties such as texture, pH, C/ N ratio, temperature, and lake physical gradient could regulate nosZII microbe abundance and diversity, and the pH and C/ N ratio may be the most important influencing factors. Studies on the ecological function of nosZII microbes have advanced considerably with the development of molecular biology technology. However, further studies on the community structure of nosZII microbes, the influencing factors of nosZII microbe abundance and diversity, and characteristics of nosZII strains with strong N_2O reducing ability are needed. We hope to provide a theoretical basis that can be used to facilitate N_2O reduction and relieve the greenhouse gas problem.
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/155100
Appears in Collections:气候变化事实与影响

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作者单位: 1.中国科学院亚热带农业生态研究所
2.中国科学院大学,
3., 长沙
4.,
5.北京 410000
6.100049
7.中国科学院亚热带农业生态研究所, 长沙, 湖南 410000, 中国

Recommended Citation:
刘春梅,魏文学,盛荣,等. 氧化亚氮还原酶基因nosZII及与环境的关系研究进展[J]. 应用与环境生物学报,2018-01-01,24(3):722-730
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