Monolepta hieroglyphica (Motschulsky)is one of the most serious pests in cotton fields in the north of Xinjiang. This study was aimed to explore the potential physiological and biochemical responses of female adult M. hieroglyphica exposed to high temperature.The variation in free amino acids, total sugar and trehalose in female adult bodies were measured under climate chamber conditions under consistent temperature regimes of 33-45℃ at 4℃ intervals for 0.5 h, 1.5 h, 2.5 h, 6 h and 12 h, respectively.Under the same heat treatment, with the extension of treatment time, the concentration of free amino acids was decreased, while the concentrations of total sugar and trehalose were increased in female adult M. hieroglyphica. For the same treatment time, with rising temperature, the concentration of free amino acids was reduced, but the concentrations of total sugar and trehalose were increased in female adult M. hieroglyphica. M. hieroglyphica even had a certain adaptability to 45℃, and thus is a serious pest to the cotton in Xinjiang under global climate warming.