The resource-environmental analysis of Jet Fuel from biomass gasification and Fischer-Tropsch synthesis (Bio-Jet Fuel) was carried out. According to the different uses of by-product steam for power generation and heat supply, two cases were studied. And the data uncertainty analysis of evaluation results was also carried out. The results show that except for eutrophication (EP), the other environmental impact indicators of the power generation case are 11.7% ~ 40.8% lower than those of the heat supply case. Compared with petroleum-based Jet fuel, the global warming impact (GWP) and depletion of non-renewable resources of Bio-Jet Fuel are reduced by 52.6%~71.9% and 84.4%~93.6%, respectively. The various distribution methods of biomass growth stage cause little influence on the resource consumption potential, but bring about some greater difference in GWP、EP. Moreover, the integrated performance of Bio-Jet Fuel is more sensitive to biomass feedstock consumption and its range of variation is -16.6% ~ +17.3%. The results of uncertainty analysis show that the uncertainties of environmental impact are between 5.0% and 12.5%.