The river basin water and ecological issues have become prominent urgent problems and have generated considerable recent research interest around the world, owing to the combined effect of global climate change and human activities. This paper first briefly presented the concept of integrated river basin management. Then we provide the concept and connotation of eco-hydrological regulation for integrated river basin management, it is synthetically guided by theory of sustainable development, theory of ecohydrology as well as nature-based solution to water issues for comprehensive contributing to dual-regulation of a river basin. With the support and participant of government and publics, especially those of mitigation measures (e.g., laws and regulations, water right and water market, ecological engineering, hydraulic engineering), it could efficiently address river basin water and ecological issues and improve benefits from water and ecosystem. Therefore, it could help to achieve a coordinated development between water resources-ecological environment-social economy, and promote the maximum of public welfare in river basin. Furthermore, we systematically elaborated the essential research contents of eco-hydrological regulation for integrated river basin management: 1) the principle and methodology of eco-hydrological regulation; 2) the integrated water resources management for eco-social coordinated and sustainable development in river basin. The results of this paper have a great implication in the enrichment and development of theoretical methods and technical systems of integrated river basin management, which would be beneficial for effectiveness as well as overall level of integrated river basin management.