The ratio of transpiration to evapotranspiration (T/ET) is a key parameter for quantifying water use efficiency of ecosystems and understanding the interaction between ecosystem carbon uptake and water cycling in the context of global change. The estimation of T/ET has been paid increasing attention from the scientific community in recent years globally. In this paper, we used the Priestly-Taylor Jet Propulsion Laboratory Model (PT-JPL) driven by regional remote sensing data and gridded meteorological data, to simulate the T/ET in forest ecosystems along the North-South Transect of Eastern China (NSTEC) during 2001-2010, and to analyze the spatial distribution and temporal variation of T/ET, as well as the factors influencing the variation in T/ET. The results show that: (1) The PT-JPL model is suitable for the simulation of evapotranspiration and its components of forest ecosystems in Eastern China, and has relatively good stability and reliability. (2) Spatial distribution of T/ET in forest ecosystems along NSTEC was heterogeneous, i.e., T/ET was higher in the north and lower in the south, with an averaged value of 0.69; and the inter- annual variation of T/ET showed a significantly increasing trend, with an increment of 0.007/yr (p < 0.01). (3) Seasonal and interannual variations of T/ET had different dominant factors. Temperature and EVI can explain around 90% (p < 0.01) of the seasonal variation in T/ET, while the inter-annual variation in T/ET was mainly controlled by EVI (53%, p < 0.05).