Previously, it was a broad consensus that transparent exopolymer particles (TEP) resulted in carbon flux output to the seabed due to its unique aggregation effect. Recently, some studies have shown that TEP could be retained in the water column and even ascend to the surface layer, which profoundly contributed to the elevated TEP concentrations and decreasing of air-sea carbon fluxes in the Surface Micro-layer (SML). The sinking or ascending of these TEP originated aggregates was finally depended on its density. TEP and further aggregated with other micro- particles became the surface-active substances in the SML, produce a thin layer film at the ocean-atmosphere interface, will heavily affect the exchange fluxes of sea-air CO_2 and further the global climate changes.