The global climate change due to the accumulation of greenhouse gases has attracted great attention.Therefore,scientists have carried out many explorations and researches on CO_2 fixation through different methods,such as chemical conversion,enzyme catalysis,and microbial transformation.The versatility of microbes has the advantage of using biomass,biological waste and carbon dioxide as raw materials to produce biofuels and chemicals.In this paper,the ways of immobilizing CO_2 naturally occurring in microorganisms were summarized,and the related work of converting carbon dioxide into chemicals or biomass energy by biological or bio-electrochemistry methods were extensively discuused.In addition,the first (using food as raw material) and second (using non-food biomass as raw material) generations of bio-manufacturing's methods and effects were evaluated,and the concept of the third generation of bio-manufacturing was introduced.Finally,the key technologies and development trends needed in the future development of carbon dioxide for biotransformation were suggested.