The classic theory of weathering limitation suggests that the chemical weathering flux is limited by the supply of fresh rocks and thus is proportionally correlated to the erosion rate as rocks were completely weathered due to the long regolith residence time at relatively low erosion rate. This is called the supply limitation. However, at relatively high erosion rate, chemical weathering flux is limited by kinetic factors such as temperature and amount of rainfall due to sufficient exposure of fresh rocks but low weathering intensity. This is called the kinetic limitation. The supply limitation is the key factor for the climate change resulted from the atmospheric CO_2 absorption in the process of silicate weathering which was influenced by the tectonic uplift. The kinetic limitation generates a negative feedback between the atmospheric CO_2 concentration and chemical weathering flux. It is the key to maintain the earth ' s habitability and balance of carbon cycle on tectonic time scale. However, the theoretical model of weathering limitation has not been sufficiently substantiated. This paper will introduce the principle and research progress for testing the weathering limitation theory using the riverine dissolved uranium isotope, (~(234)U/~(238)U) Riv. A review of global data indicates that there is a generally U-shaped functional relationship between the (~(234)U/~(238)U) Riv and catchment scale denudation rate (D). This can be explained by the classic weathering limitation theory. However, the (~(234)U/~(238)U) Riv is also complicatedly controlled by other factors including lithology, climate, landscape and so on. It would be an important way to verify the weathering limitation theory by using the riverine uranium isotope of a single lithological drainage basin with known weathering age.