Agricultural ecosystem is the most important emission source of atmospheric greenhouse gas (GHG) and its production amount accounts for 10% to 12% of the total global anthropogenic GHG. Cropland management influences GHG emissions, which can be reduced by adopting conservation practices, such as inhibitor applications, coated slow-released fertilizer additions, tillage practices, irrigation, residue management, and so on. GHG reduction from agricultural ecosystem plays a positive role in mitigating global warming. Therefore, a winter wheat field in Pingdingshan city, Henan province were selected as the research object. Five kinds of nitrogen (N) application measures, i.e., urea+urease inhibitor (U+HQ), urea+nitrification inhibitor (U+DCD), urea+urease inhibitor+nitrification inhibitor (U+HQ+DCD), polymer coated urea (PCU) and urea (U) as the control, were set up. Nitrous oxide (N_2O), carbon dioxide (CO_2) and methane (CH_4) fluxes, as well as their cumulative amounts were analyzed in the main cultivation stages of winter wheat by using the static opaque chamber-gas chromatogram techniques. The results showed that three kinds of greenhouse gases fluxes had seasonal fluctuation, tended to decrease first then increase and final decrease. Compared with U, the other four N application measures all reduced cumulative N_2O flux, tended to U+DCD>PCU>U+HQ>U+HQ+DCD, among which U+DCD and PCU had significantly mitigated its emission, decreased by 44.44% and 20.99% (P<0.05). Moreover, U+DCD, U+HQ+DCD and PCU except for U+HQ had significantly reduced the cumulative CO_2 flux (P<0.05), the mitigation rates tended to U+HQ+DCD (42.29%)>PCU (40.84%)>U+DCD (34.78%)>U+HQ (6.07%). Compared with U, the other four N application measures also significantly reduced cumulative CH_4 absorption (P<0.05), among them, U+DCD and U+HQ+DCD decreased significantly by 48.21% and 49.40% (P<0.05). Compared with U, the other four N application measures all significantly reduced global warming potential, the mitigation rates were as follow, PCU>U+HQ+DCD>U+DCD>U+HQ, decreased by 38.29%, 36.64%, 36.03% and 6.54% (P<0.05), respectively. Wheat yields were impacted by different N application measures (P<0.05), tended to U+DCD>U+HQ>U>PCU> U+HQ+DCD. Comprehensively, common urea combined with nitrification inhibitor can effectively mitigate greenhouse gas emissions and increase winter wheat yield.