Greenhouse gas emission reduction has been one of the research hotspots under the context of global climate change. The effects of moisture content and C/N coupling mode on reducing greenhouse gas emission and global warming potential (GWP) were studied in this paper., so as to find the suitable moisture content and C/N coupling mode for reducing greenhouse gas emission, and provide the references or reasons for greenhouse gas emission reduction in manure composting. The six donkey manure composting treatments, such as W_1R_1(water content: 55%; C/N: 25:1), W_1R_2 (water content: 55%; C/N: 30:1), W_1R_3 (water content: 55%; C/N: 35:1), W_2R_1 (water content: 60%; C/N: 25:1), W_2R_1 (water content: 60%; C/N: 30:1) and W_2R_3 (water content: 60%; C/N: 35:1) were set to research greenhouse gas emissions and global warming potential by using the static chamber-gas chromatography. The results showed that the manure temperature increased in the early stage and decreased in the mid and later stage, but the opposite trend happened to the oxygen content, so the manure temperature and oxygen content showed a significantly negative correlationship. Lower C/N ratio and water content were conducive to the manure temperature increasing. The N_2O, CH_4, and CO_2 emission fluxes mainly increased in early period and decreased in mid and later stage, and the biggest emission fluxes of N_2O, CH_4, and CO_2 appeared around 24 d, 8 d and 16 d after manure composting, respectively. The CH_4 and CO_2 emission fluxes positively correlated with the manure temperature among the six composting modes, but the significantly negative relationship happened to CH_4 emission flux with the manure oxygen content. GWP decreased in sequence of W_2R_1>W_2R_3>W_1R_2>W_1R_1>W_2R_2>W_1R_3. The cumulative CO_2 emission amount of each treatment made the greatest contribution to GWP, the contribution proportion was more than 66.30%, N_2O contribution proportion varied from 2.07% to 32.45%, and CH_4contribution proportion was less than 1.54%. Considering the manure composting temperature and global warming effect, the W_1R_3 composting mode should be recommended.