The changes of glomalin-related soil protein (GRSP) are important indicators for evaluating and indicating the dynamic changes of soil carbon (C) pool. Understanding the GRSP change mechanism under the background of increasing atmospheric nitrogen (N) deposition is of great significance to elucidate the driving factors of soil C cycle. Therefore, this study conducted field control experiments on the experimental sample site located in Greater Khingan Mountains to explore the influence mechanism of GRSP on N deposition. In the test sample, the N addition treatment was started in May 2011, and a total of four levels of N addition treatment were set, which were control (Control, 0 g·am~(-2)·a~(-1)), low N (LN, 2.5 g·m~(-2)·a~(-1)), medium N (MN, 5 g·m~(-2)·a~(-1)) and high N (HN, 7.5 g·m~(-2)·a~(-1)) treatment. The results showed that, (1) Easy extract glomalin (EE-GRSP) increased in soil of all N levels, and total glomalin (T-GRSP) in LN and MN treatment was significantly promoted (P<0.05), but it showed inhibition in HN treatment. (2) There was a significant positive correlation between the contents of T-GRSP and EE-GRSP and soil organic matter (SOM) (P<0.05). The contribution rate of GRSO to SOM increased by 0.59%-1.07% with N application. Low and medium level N deposition (LN and MN) significantly promoted the accumulation of SOM (P<0.05), while high level N deposition (HN) was inhibited. In addition, the contribution rate of GRSP to SOM of deciduous pine forest in Greater Khingan Mountains was relatively low. And (3) in the context of climate change, low and medium level N deposition could increase the accumulation of SOM by increasing the amount of GRSP, while high level N deposition could reduce the total SOM of soil by reducing the amount of GRSP. This indicates that the low and medium level N deposition can increase GRSP and SOM content, thereby improving the potential of solid C in the ecosystem and alleviating the pressure caused by the increase of atmospheric CO_2 concentration.