Grapholita molesta (Busck) is a worldwide pest of fruit trees, and overwinters as mature larvae. The suitable growing area of host plants has been enlarged with the new variety breeding of fruit trees and climate warming. In order to determine the cold resistance of G.molesta, or is it possible to migrate to the cold areas following the enlargement of suitable growing area of host plants. The supercooling points and freezing points of different development stages, larval instars, sex of pupae and adults, and conditions were measured, so as to judge whether they could overwinter successfully or not. The results showed that there was no significant difference in the supercooling points and freezing points for the two sexes of pupae and adults, and the supercooling points and freezing points differed among different developmental stages and larval instars, and larval conditions. The ranking of the supercooling points and freezing points for different developmental stages was as followed: larva>pupa>adult. The supercooling point (-12.470.27)℃ and freezing point (-8.210.41)℃ of 5th instar larvae were lowest, whereas the supercooling point (-9.230.38)℃ and freezing point (-5.060.37)℃ of 4th instar larvae were highest. When compared with indoor larvae without cold acclimation, outdoor larvae had a lower supercooling point (-24.420.69)℃ and freezing point (-10.210.71)℃, but the supercooling point of indoor larvae could be reduced by cold acclimation. Therefore, our results indicated that the cold resistance of mature larvae was strong, and it could be improved by cold acclimation.