Based on the daily data from 1961 to 2010 collected from 20 meteorological stations in Zhejiang Province,the spatiotemporal characteristics of agricultural climate resources were analyzed. It was shown that annual mean temperature exhibited a significant increasing trend at 0.27℃ per decade. The accumulated temperature above 10℃ and temperature-defined growing period were also increased, and the terminate date of 20℃ was delayed. The spatial distributions of heat resource presented a downward trend from north to south. The average sunshine hour was larger in the north than that in the south. During 1961-2010, sunshine hours showed a significant decreasing trend, especially in Jinqu Basin and Taizhou. The annual precipitation increased, while the rainy days decreased, which indicated that the rainfall intensity increased. ET0 analysis presented an obvious annual change characteristic of "decrease-little fluctuation-increase". According to the spatial distribution, the high-value center was located in the inland area and Jinqu Basin, while low-value area was located in Lishui. The mean humid index during 1961-2010 was 1.64 with an increasing trend. The present research would provide reference for planting pattern adjustment to cope with climate change in Zhejiang Province.