In order to improve the utilization rate of agroclimatic resource, reduce the risk of agricultural production and ensure food safety, the spatial and temporal distribution characteristics of agroclimatic resource during main crops growing season in the south of Huaihe River Basin were analyzed by using daily meteorological data of 33 stations from 1971 to 2005. Results of Mann-Kendall test and climatic trend analysis showed that, (1) In recent 45 years, the heat resources of main crops growing season were both more in the north than the south and in the mountain areas than the plain areas. In the northern, eastern and part of western of Huaibei the heat resources increased slowly, while in parts of northwest and northeast of Huaibei as well as middle area along the Huaihe River the heat resources increased significantly. The warming trend of winter wheat (Triticum aestivum Linn) growing season was more significant in the late 1990s, while that of summer corn (Zea mays L) and rice (Oryza sativa L) growing season reached a very significant level in the middle of the 2000s. (2) The first frost date was early in the north and late in the south as well as early in the plain area and late in the mountain area. The first frost date came later by the years. However, the spatial and temporal distribution of the last frost date was the opposite. Therefore, the frostless period was prolonged. The trends of delay of the first frost day and advance of last frost day were more significant at the beginning of the 2000s. (3) The precipitation of main crops growing season showed obvious zonal distributions and the north was much less than the south. The climatic trend rate increased or decreased slightly, which did not show significant trend but fluctuated greatly. The difference of precipitation variability in winter wheat growing season was not obvious everywhere except the mountainous area, where was obviously small. The precipitation variability increased before 2000, while it was about 15% in most of the years after 2000.The precipitation variability in summer corn and rice growing season was higher in the west than the middle east and higher in the plain areas than the mountain areas, which increased before 2005 then decreased. And (4) the radiation resources were more in the north than the south and less in the west and mountain areas. The radiation of most stations showed decreasing trend, and the decreasing rate in the western region was higher than that in other regions. The downward trend of radiation resources in winter wheat growing season was not significant, which varied little between generations. However, solar radiation resources in summer corn and rice growing season decreased significantly and exhibited even more significantly decreasing trend after the 2000s.