The experimental Chlorella of Chlorella pyrenoidosa FACHB-5 and Chlorella vulgaris FACHB-8 was cultured in a 1 000 mL Erlenmeyer flask with 700 mL nutrient solution in an artificial climate incubator. The culture temperature, illuminance, airflow rate and continuous illumination time were (261) ℃, 4 000 lx, 1.5 L/min and 24 h, respectively. The biogas slurry of chicken manure achieved after anaerobic digestion at medium temperature (35 ℃) and high temperature (55 ℃) for 45 d was used as nutrient source for Chlorella culture. The main compositions and characteristics of biogas slurry digested at 35 and 55 ℃ were 2 417.63 and 2 554.37 mg/L for total nitrogen, 2 218.27 and 2 376.24 mg/L for ammonium nitrogen, 16.91 and 13.44 mg/L for total phosphorus, 10 021.82 and 10 104.13 mg/L for chemical oxygen demand (COD), 8.25 and 8.18 for pH value, 698 and 653 NTU for turbidity, and 2.68 and 2.52 mPa·s for viscosity, respectively. The semi-continuous culture of Chlorella was realized by discharging nutrient solution of 10% and adding new nutrient solution of 10% at the same time daily. Furthermore, the addition ratios of unsterilized biogas slurry in daily new nutrient solution were 10%, 20% and 30%, respectively. Effects of 3 different addition ratios of unsterilized biogas slurry digested at 35 and 55 ℃ on the semi-continuous culture of Chlorella were studied systematically based on the analysis of nutrient solution during Chlorella culture including OD_(680), total nitrogen, ammonium nitrogen, total phosphorus, and so on. The results showed that the Chlorella pyrenoidosa FACHB-5 and Chlorella vulgaris FACHB-8 could adapt the culture environment well and grow rapidly when the addition ratios of unsterilized biogas slurry in daily new nutrient solution were 10% and 20%. Furthermore, the OD_(680) values of 2 types of Chlorella were maintained at 2.40-2.69 at the end of Chlorella culture for 10 d. However, the growth of FACHB-5 and FACHB-8 in the later period of culture experiment was restrained significantly when the addition ratio of unsterilized biogas slurry in daily new nutrient solution was 30%. Furthermore, the growth rates and adaptive capacity of FACHB-5 in the nutrient solution containing more biogas slurry were better than that of FACHB-8. A similar fluctuation trend was achieved in OD_(680) of different comparative samples with biogas slurry addition. However, the OD_(680) values of comparative samples with 55 digestive biogas slurry were over 0.13 higher than that of comparative samples with 35 digestive ℃ ℃ biogas slurry, especially for the comparative samples with biogas slurry of 10% and 20% addition ratios. Moreover, the OD_(680) values of FACHB-5 and FACHB-8 were maintained at 1.98-2.15 and 1.79-1.92 at the end of Chlorella culture for 10 d, respectively. Therefore, the effects of biogas slurry digested at 55 ℃ on semi-continuous culture of Chlorella were superior than that of biogas slurry digested at 35 ℃. The results supply references for the theoretical research and subsequent large-scale application of Chlorella culture using biogas slurry digested at different temperatures.