The MAT_(mr) records of Glycerol Dialkyl Glycerol Tetraether lipids (GDGTs) from Guanjiashan section (36°40.55'N, 101°50.24'E), Xining Basin, NE Tibetan Plateau, suggest the continuous cooling between 16.8 Ma and 13.4 Ma (droping in temperature about 5.1 ℃). Our result is consistent with the climatic events responding to Middle Miocene Climate Optimum (MMCO) and Middle Miocene Climate Transition(MMCT) recorded by global deep-sea oxygen isotope records. Thus, it reveals that MAT_(mr) were controlled by global temperature change during this period. The mean value of CBT'-derived paleo-pH (CBT'-pH) values is 6.560.50,and the abundances of bGDGTs (branched GDGTs) relative to that of archaeal isoprenoidal(iso) GDGTs (R_(i/b),as a drought proxy in Chinese soils) for 80% samples are less than 0.5, together indicating relatively humid in that period as a whole in the Xining Basin. But two drought events were recorded by R_(i/b) values in 14.0 Ma and 14.8 Ma. Although it's difficult to estimate the absolute paleo-temperature at present, GDGTs have important potentiality to reconstruct paleo-temperature of Xining Basin, and so far as to NE Tibetan Plateau since Neogene and even older sediments.