DOI: 10.1007/s12038-020-9996-x
论文题名: Comprehensive molecular insights into the stress response dynamics of rice (Oryza sativa L.) during rice tungro disease by RNA-seq-based comparative whole transcriptome analysis
作者: Kumar G. ; Dasgupta I.
刊名: Journal of Biosciences
ISSN: 2505991
出版年: 2020
卷: 45, 期: 1 语种: 英语
英文关键词: Differentially expressed genes
; pathway analysis
; rice tungro disease
; RNA-Seq
; transcriptome
Scopus关键词: ascorbic acid
; cytochrome P450
; ferredoxin
; glutaredoxin
; glutathione peroxidase
; heat shock protein 100
; heat shock protein 40
; heat shock protein 70
; homeodomain protein
; oxidoreductase
; peroxiredoxin
; reduced nicotinamide adenine dinucleotide phosphate oxidase
; superoxide dismutase
; thioredoxin
; comparative study
; crop yield
; gene expression
; homeostasis
; molecular analysis
; rice
; RNA
; stress resistance
; Article
; biosynthesis
; cell transport
; comparative study
; controlled study
; copy number variation
; down regulation
; gene expression regulation
; gene ontology
; homeostasis
; hormonal homeostasis
; innate immunity
; molecular diagnosis
; nonhuman
; paired end sequencing
; plant disease
; plant immunity
; plant stress
; protein localization
; quantitative analysis
; real time polymerase chain reaction
; rice
; Rice tungro bacilliform virus
; rice tungro disease
; Rice tungro spherical virus
; RNA sequence
; sequence alignment
; signal transduction
; upregulation
; validation study
; virus detection
; whole transcriptome sequencing
; Oryza sativa
; Rice tungro bacilliform virus
; Rice tungro spherical virus
英文摘要: Rice tungro is a serious viral disease of rice resulting from infection by two viruses, Rice tungro bacilliform virus and Rice tungro spherical virus. To gain molecular insights into the global gene expression changes in rice during tungro, a comparative whole genome transcriptome study was performed on healthy and tungro-affected rice plants using Illumina Hiseq 2500. About 10 GB of sequenced data comprising about 50 million paired end reads per sample were then aligned on to the rice genome. Gene expression analysis revealed around 959 transcripts, related to various cellular pathways concerning stress response and hormonal homeostasis to be differentially expressed. The data was validated through qRT-PCR. Gene ontology and pathway analyses revealed enrichment of transcripts and processes similar to the differentially expressed genes categories. In short, the present study is a comprehensive coverage of the differential gene expression landscape and provides molecular insights into the infection dynamics of the rice-tungro virus system. © 2020, Indian Academy of Sciences. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158051
Appears in Collections: 气候变化与战略
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作者单位: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110 021, India
Recommended Citation:
Kumar G.,Dasgupta I.. Comprehensive molecular insights into the stress response dynamics of rice (Oryza sativa L.) during rice tungro disease by RNA-seq-based comparative whole transcriptome analysis[J]. Journal of Biosciences,2020-01-01,45(1)